Labor is not only a fine traditional virtue of the Chinese nation, but also the practical foundation of Marxist worldview. Labor education is the core content of Marxist educational thought, one of the essential characteristics of socialist education, and the internal requirement of comprehensively implementing the educational policy of the CPC. This study adheres to the guidance of Marxism, takes the new era as the research background, and takes the group of applied undergraduate students in the new era represented by Pass College of Chongqing Technology and Business University as the main research object. It analyzes the reasons for the problems from aspects such as school education, social impact, family education, and self-awareness. Based on comprehensive research data, it can be seen that college students in application-oriented undergraduate universities represented by Pass College of Chongqing Technology and Business University, have a sense of participation in labor practice, and actively learn labor knowledge through a combination of traditional and emerging channels. They are able to learn and practice labor values in labor education practice, and actively accept and participate in various types of labor education organized by university organizations. The research results indicate that in the new era, applied undergraduate universities should strengthen labor education by keeping up with the development of the times, strengthening labor model demonstration, creating an open ecosystem of labor education, constructing a multi-module labor education curriculum structure and form, and promoting the integration of labor education and campus culture construction. Through this study, innovative ways of implementing labor education in applied undergraduate universities in the new era can be explored, and innovative paths for strengthening labor education in applied undergraduate universities in the new era can be explored.