Endophytic fungi from tropical medicinal plants are microbial resources with significant bioactivity. They can co-evolve with their hosts over long periods and produce compounds with novel structures. These fungi hold considerable application value in preventing and controlling agricultural plant fungal diseases and treating clinical drug-resistant bacterial infections. This study focuses on endophytic fungi from tropical medicinal plants, elucidating their chemical structure types and conducting in-depth analyses of the biosynthetic pathways, key enzyme systems, and regulatory mechanisms of gene clusters for fungicidal active products. It systematically explores the molecular mechanisms of fungicidal action and analyzes indicators such as the diameter of inhibition zones and minimum inhibitory concentrations. This research provides theoretical foundations and data references for the efficient exploration of fungicidal active substances from endophytic fungi in tropical medicinal plants, optimization of biosynthesis, and development of novel natural fungicides.