Extracellular Trap Release and Inflammation Induction in Mast Cells by Fusobacterium nucleatum

Hiroyuki Tada; Takashi Nishioka; Kenji Matsushita; Sakura Onoue; Kazuyoshi Kawahara

doi:10.18063/amcmr.v1i2.0030

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Volume 6,Issue 3

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Extracellular Trap Release and Inflammation Induction in Mast Cells by Fusobacterium nucleatum

Hiroyuki Tada^1*, Takashi Nishioka², Kenji Matsushita³, Sakura Onoue⁴, Kazuyoshi Kawahara⁴

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¹ Division of Oral Immunology, Graduate School of Dentistry, Tohoku University, Sendai, Miyagi 980-0872, Japan

² Division of Oral Diagnosis, Graduate School of Dentistry, Tohoku University, Sendai, Miyagi 980-0872, Japan

³ Department of Oral Disease Research, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8511, Japan

⁴ Department of Biosciences, College of Science and Engineering, Kanto Gakuin University, Kanazawa, Yokohama 236-0037, Japan

CBR 2020 , 1(1), 15–19; https://doi.org/10.18063/amcmr.v1i2.0030

© 2020 by the Author(s). Licensee Whioce Publishing, Singapore. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution -Noncommercial 4.0 International License (CC BY-NC 4.0) ( https://creativecommons.org/licenses/by-nc/4.0/ )

Abstract

Mast cells play an important role in the innate immune responses to bacterial infections as the first line of defense such as in the skin and mucosa. Mast cells can produce extracellular traps to kill bacteria by trapping pathogens. Mast cell-extracellular traps (MCETs) are composed of web-like DNA fibers that contain bactericidal substances such as DNA, histones, tryptase, and antimicrobial peptides. At present, it is unknown whether the induction of inflammation in periodontal diseases is due to MCETs induced by periodontal bacteria. We investigated the role of mast cells in the induction of MCET production following infection with Fusobacterium nucleatum, a gramnegative anaerobic bacterium associated with periodontal disease. We found that mast cells produced MCETs in response to F. nucleatum infection. Furthermore, the MCETs highly expressed macrophage migration inhibitory factor (MIF). Of note, the level of MIF expressed in the MCETs was inhibited by taurolidine, an LPS antagonist. We next investigated whether MCETs can induce inflammatory responses in monocytes. The MCETs induced the production of IL-1β, IL-6, and IL-8 by monocytes. The production of IL-1β, IL-6, and IL-8 was inhibited by an MIF inhibitor. These findings suggest that MCETs produced by mast cells in response to F. nucleatum infection induce proinflammatory cytokine production by monocytes, which may lead to the chronic inflammation observed in periodontal diseases.

Keywords

Chronic periodontitis

Mast cells

Mast cell-extracellular traps

Fusobacterium nucleatum

Lipopolysaccharide

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Conflict of interest

The authors declare no conflicts of interest.

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