Volume 10,Issue 1
Fall 2025
Clinical Effectiveness of Respiratory Virus Detection by FilmArray Method in Children Admitted with Respiratory Infection
Purpose: Respiratory virus infection is a common cause of hospitalization in children. Rapid testing for respiratory viruses, such as the FilmArray method, can be clinically useful. However, insufficient evidence exists to support its use in standard clinical care. Methods: We retrospectively analyzed data from children under 18 years old who received the multiplex real-time polymerase chain reaction array (multiplex RT-PCR) method in 2017 and by FilmArray respiratory panel (FilmArray RP) in 2018. Results:Between January 2017 and December 2018, we reviewed data from 1,480 hospitalized children. The number of children with virus detection in respiratory viral PCR was 523 in the multiplex RT-PCR method and 419 in the FilmArray method. Seasonal virus outbreak patterns were similar to those of the Korea Centers for Disease Control and Prevention in both groups. There was no difference between the 2 groups in the mean length of hospital stay. The time from admission to isolation by influenza infection was significantly shorter in the FilmArray group than in the multiplex RT-PCR group among patients who were not diagnosed with influenza infection by rapid antigen test at the time of admission.Conclusion: The use of the FilmArray method for respiratory viruses did not diminish the length of hospital stay. However, the FilmArray method may quickly detect the prevalence of respiratory infection and aid in clinical treatment. In addition, it was related to a reduced time from admission to isolation by influenza infection in hospitalized children who were not identified with influenza infection by rapid antigen test at the time of admission.
1. Global Health Observatory, Causes of Child Mortality, 2017. World Health Organization, 2017. Viewed 7 April 2020, https://www.who.int/gho/child_health/mortality/causes/en
2. Williams BG, Gouws E, Boschi-Pinto C, et al., 2002, Estimates of World-Wide Distribution of Child Deaths from Acute Respiratory Infections. Lancet Infect Dis, 2: 25–32.
3. Lee GE, Lorch SA, Sheffler-Collins S, et al., 2010, National Hospitalization Trends for Pediatric Pneumonia and Associated Complications. Pediatrics, 126: 204–213.
4. Thornton HV, Blair PS, Lovering AM, et al., 2015, Clinical Presentation and Microbiological Diagnosis in Paediatric Respiratory Tract Infection: A Systematic Review. Br J Gen Pract, 65: e69–e81.
5. Ma XL, Conrad T, Alchikh M, et al., 2018, Can We Distinguish Respiratory Viral Infections Based on Clinical Features? A Prospective Pediatric Cohort Compared to Systematic Literature Review. Reviews in Medical Virology, 28: e1997.
6. Infectious Diseases Surveillance Yearbook. Korea Centers for Disease Control and Prevention, 2020. Viewed 7 April 2020, http://www.cdc.go.kr/npt/biz/npp/portal/nppPblctDtaView.do? pblctDtaSeAt=1&pblctDtaSn=1873
7. Hanson KE, Couturier MR, 2016, Multiplexed Molecular Diagnostics for Respiratory, Gastrointestinal, and Central Nervous System Infections. Clin Infect Dis, 63: 1361–1367.
8. Brendish NJ, Malachira AK, Armstrong L, et al., 2017, Routine Molecular Point-of-Care Testing for Respiratory Viruses in Adults Presenting to Hospital with Acute Respiratory Illness (ResPOC): A Pragmatic, Open-Label, Randomised Controlled Trial. Lancet Respir Med, 5: 401–411.
9. Rappo U, Schuetz AN, Jenkins SG, et al., 2016, Impact of Early Detection of Respiratory Viruses by Multiplex PCR Assay on Clinical Outcomes in Adult Patients. J Clin Microbiol, 54: 2096–2103.
10. Reischl AT, Schreiner D, Poplawska K, et al., 2020, The Clinical Impact of PCR-Based Point-of-Care Diagnostic in Respiratory Tract Infections in Children. J Clin Lab Anal, 34: e23203.
11. Acute Respiratory Infection. Korea Centers for Disease Control and Prevention, 2020. Viewed 7 April 2020, http://www.cdc.go.kr/npt/biz/npp/iss/ariStatisticsMain.do
12. Lee J, Lee HS, Cho YG, et al., 2018, Evaluation of Allplex Respiratory Panel 1/2/3 Multiplex Real-Time PCR Assays for the Detection of Respiratory Viruses with Influenza A Virus Subtyping. Ann Lab Med, 38: 46–50.
13. Rogers BB, Shankar P, Jerris RC, et al., 2015, Impact of a Rapid Respiratory Panel Test on Patient Outcomes. Arch Pathol Lab Med, 139: 636–641.
14. Renaud C, Crowley J, Jerome KR, et al., 2012, Comparison of FilmArray Respiratory Panel and Laboratory-Developed Real-Time Reverse Transcription-Polymerase Chain Reaction Assays for Respiratory Virus Detection. Diagn Microbiol Infect Dis, 74: 379–383.
15. Poritz MA, Blaschke AJ, Byington CL, et al., 2011, FilmArray, an Automated Nested Multiplex PCR System for Multi-Pathogen Detection: Development and Application to Respiratory Tract Infection. PLoS One, 6: e26047.
16. Garcia-Garcia ML, Calvo C, Pozo F, et al., 2012, Spectrum of Respiratory Viruses in Children with Community-Acquired Pneumonia. Pediatr Infect Dis J, 31: 808–813.
17. Jain S, Williams DJ, Arnold SR, et al., 2015, Community-Acquired Pneumonia Requiring Hospitalization Among U.S. Children. N Engl J Med, 372: 835–845.
18. An SH, Cho HJ, Baek HS, et al., 2018, Clinical Features of Mycoplasma pneumonia in Comparison with Viral Pneumonia in Children: A Multicenter, Cross-Sectional Study. Allergy Asthma Respir Dis, 6: 155–160.
19. Pavia AT, 2011, Viral Infections of the Lower Respiratory Tract: Old Viruses, New Viruses, and the Role of Diagnosis. Clin Infect Dis, 52 Suppl 4: S284–S289.
20. Kim HY, Kim KM, Kim SH, et al., 2012, Clinical Manifestations of Respiratory Viruses in Hospitalized Children with Acute Viral Lower Respiratory Tract Infections from 2010 to 2011 in Busan and Gyeongsangnam-do, Korea. Allergy Asthma Respir Dis, 22: 265–272.
21. Lee SJ, Lee SH, Ha EK, et al., 2017, Prevalence of Respiratory Virus Infection with Regard to Age, Sex, and Seasonality Factors: A Single Center Experience Against Children Hospitalized During the 10 Years. Allergy Asthma Respir Dis, 5: 320–325.
22. Infectious Diseases Surveillance Yearbook. Korea Centers for Disease Control and Prevention, 2017, Cheongju, 363–365.
23. Infectious Diseases Surveillance Yearbook. Korea Centers for Disease Control and Prevention, 2018, Cheongju, 361–363.
24. Busson L, Bartiaux M, Brahim S, et al., 2019, Contribution of the FilmArray Respiratory Panel in the Management of Adult and Pediatric Patients Attending the Emergency Room During 2015-2016 Influenza Epidemics: An Interventional Study. Int J Infect Dis, 83: 32–39.
25. Echavarria M, Marcone DN, Querci M, et al., 2018, Clinical Impact of Rapid Molecular Detection of Respiratory Pathogens in Patients with Acute Respiratory Infection. J Clin Virol, 108: 90–95.
26. Moodley A, Bradley JS, Kimberlin DW, 2018, Antiviral Treatment of Childhood Influenza: An Update. Curr Opin Pediatr, 30: 438–447.
27. Wang K, Shun-Shin M, Gill P, et al., 2012, Neuraminidase Inhibitors for Preventing and Treating Influenza in Children (Published Trials Only). Cochrane Database Syst Rev, 2012: CD002744.
28. Jefferson T, Jones MA, Doshi P, et al., 2014, Neuraminidase Inhibitors for Preventing and Treating Influenza in Healthy Adults and Children. Cochrane Database Syst Rev, 2014: CD008965.